Biometra t3000 thermocycler manual

Make sure that the ventilation slots on the bottom and the rear are not obstructed see section 3. There must be sufficient distance between the ventilation slots on the rear side of the Thermocycler and a wall or another instrument min 10 cm.

Make sure that there is no object underneath the thermocycler that may block the ventilation slots on the bottom e. Make sure that the main supply voltage is in accordance with the label above the power connection see section 3. Please keep the original packaging material for return shipment in case of servicing.

The T carton provides a specially developed system for contact-free transport of this electronic device. The display contrast can be adjusted to local lightning conditions see section 8. Before connecting the unit to the power source please ensure that the voltage selector at the back side of the instrument is set to the required voltage.

Danger of electric shock! Unplug the power cable before you open the T Thermocycler. Ensure that both the rear and bottom ventilation slits of the rear and bottom of the instrument are unobstructed. Insufficient ventilation can cause overheating of the instrument. Ventilation slots Power switch. Block status Graphical LED display. After switching on the T the serial number of the instrument and the software version is displayed.

Serial number Block type Vers. The T display provides information about the instrument and about the status of the active program. Prog Step Temp The function of the four soft keys beneath the display is context sensitive. The respective function is shown in the display. The function of each key can differ from screen to screen. The T thermocyclers provides spread sheet programming. This means that all program parameters are entered into a simple central spreadsheet. Four navigation keys provide easy navigation within the software.

This cursor can also be used to complete data entry. By pressing the right cursor settings will be saved and the cursor moves to the next field. In the file directory, this key moves to cursor forward to the next lower level. To achieve optimum pressure on the tubes the T is equipped with an height adjustable heated lid.

Close the lid: After the samples have been placed in the block close the lid. Turn the wheel clockwise until you hear a clicking noise. In this mode the pressure will not increase further, even when you keep on turning the wheel. Note: The pressure of the lid has been optimise for a fully loaded block. If only very few tubes are loaded to the block you should place dummy tubes in the four corner positions to avoid damage of tubes by excessive pressure.

Open the heated lid: First: Release pressure by turning the wheel counter clockwise. As soon as there is no more resistance the pressure has been released. Then: now you can open the lid with the knob. Important: The lid should not be opened under pressure because this leads to damage of the locking mechanism. Note: When the lid is in the extreme up or down position, it may happen that the wheel is uncoupled.

In this situation the clutch mechanism is active in both directions clicking noise in either direction. To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin overcomes the automatic clutch mechanism.

Thus, care must be taken not to apply excessive pressure. Release pin and turn lid down, until the clutch mechanism is activated clicking noise, optimum pressure applied. Release pin and turn wheel counter clockwise until pressure is completely released. Open lid. The T Thermocycler provides 10 subdirectories of which each can store up to 99 different programs. For easy identification of the selected storage place, the subdirectories can be named see section. Each program is specified by a program number and a subdirectory number.

To make retrieval of a program easier, you can enter a name for each program existing of letters, numbers and symbols. Blank Del. Name OK Enter. Use cursor keys to select a letter. Lid temp C Preheating On. Note: In general, the lid temperature should be about 10C above the highest temperature in the protocol. Example: If your denaturation step is at 99C, enter C for the lid. Lid temp You can choose whether the lid is pre-heated before the program starts.

This is to avoid condensation during the initial heating phase. Note: In this spreadsheet you can enter all parameters for your cycling protocol. In addition, you can set special parameters like touch down, ramping rates or time increments. For details on these special parameters, see section 5. You can navigate in the spreadsheet without limitations with the four cursor keys.

Note: Each setting is confirmed with [Enter]. The cursor moves automatically to the next field. Alternatively, you can confirm a value by moving forward with the cursor keys.

Note: In an existing protocol program steps can be deleted and inserted. For further information on deleting and inserting program steps see section 5. Now enter temperature for the first step an press [Enter]. In the next row you can enter the time for this temperature:.

To program minutes enter a l after the number of minutes. To enter hours enter l l after the number. You can also enter any combination of hours, minutes and seconds. Example: for 1 hour, 30 minutes, 20 seconds enter 1l 30 l Note: In general, loops are defined by selecting the target for the back loop and the number of backloops.

In the row labelled with enter the target step for a backloop and press [Enter]. In the row labelled with you can enter the number of backloops. This protocol now contains 25 cycles between step 2 To hold the temperature for an indefinite time enter 0. After pressing [Enter] pause is displayed. Save program with [Save Pgm]. Note: The run time is calculated according to the block type. Please be aware that different block types provide different ramping rates see section 10, Technical specification.

Note: To view the remaining runtime during a run press [Info] in the main menu. Note: During operation of the cycler the active program can be viewed but not modified.

If you want to change settings of the active program for further experiments you have to save a copy of this program to another memory. For further information about copying programs see section 5. Cancel Delete Insert. Note: The new step has been entered within an existing loop, this loop now consists of three steps.

Note: Additional steps will be inserted before the selected step. The following steps are shifted by one position. Note: The insertion and deletion of steps may have an influence on existing loops. Be sure that all settings are updated to maintain a correct cycle. Select program as described in sections 4. Note: To program special parameters you can scroll the display to the right. Move the cursor to the right side row of the display Options. Main funct. Note: To go back to programming sheet, move the cursor to the very left side of the display row Main funct.

Note: In the main screen you will find an information that a special parameter has been modified. To compensate for loss in enzyme activity each step within a loop can be extended from cycle to cycle. Enter the desired time increment [seconds] in the row labelled with dt[s]. This value will be added to the time value from cycle to cycle.

Note: A time increment will have an impact on the total runtime depending on the numbers of cycles and the size of the increment. A program with many cycles and large time increments will take a significantly longer time than a standard protocol. For some applications it is useful to start with a higher annealing temperature and to decrease the temperature from cycle to cycle. Make sure that the appliance connector and the plug of the supplycord are accessible, so you can separate the instrument from themains.

Connect the T Thermocycler to a grounded socket. When only few samples are put in the block place additional tubes inthe four corner positions. This is to evenly distribute the lid pressureand prevents single tubes from excessive pressure.

Use of few tubesmay result in damage of the tubes by excessive pressure. Appropriate safety regulations must be observed when working withinfectious, pathogenic or radioactive material. Ask the responsiblelocal safety inspector for details. The T Thermocycler must not be used with explosive,flammable or volatile liquids.

Do not place fingers between lid and housing when opening orclosing the lid. Before opening of the lid, release lid pressure see section 4. If you still decide to use oil, do not use silicon oil.

Mineral oil may beused. Ensure that both the rear and bottom ventilation slits not clogged bydust or other material.

Danger of overheating! Let equilibrate the T Thermocycler to room temperature beforestarting operation. There must be sufficient distance between the ventilation slots on theside of the Thermocycler and a wall or another instrument min 10cm.

This instrument is designed and certified to meet EN safetystandards. It should not be modified or altered in any way. Alterationof this instrument will void the warranty, void the ENcertification, and create a potential safety hazard. Place the T Thermocycler on a stable, non flammable surface ina dry, safe environment. Do not use alcohol e.

For transports always use the original Biometra box. The Tshipping box provides a specially developed system for contact-free transport of this electronicdevice.

Report any damage to Biometra. Do not attemptto operate this device if physical damage is present. Please keep the original packing material for return shipment in case of service issues!!

Please fill out and send back the warranty registration card. This is important for you toclaim full warranty. Make sure that there is no object underneath the thermocycler that may blockthe ventilation slots at the bottom e. Ensure that both the side and bottom ventilation slits of theside and bottom of the instrument are unobstructed.

Insufficient ventilation can cause overheating of theinstrument. T ThermocyclerSerial numberBlock typeVers. Close the lid:After the samples have been placed in the block close the lid. Turn the wheel clockwise untilyou hear a clicking noise. In this mode the pressure will not increase further, even when youkeep on turning the wheel.

Note: The pressure of the lid has been optimized for a fully loaded block. If only very few tubesare loaded to the block you should place dummy tubes in the four corner positions to avoiddamage of tubes by excessive pressure.

Open the heated lid:First: Release pressure by turning the wheel counter clockwise. As soon as there is no moreresistance the pressure has been released. Then: Now you can open the lid with the knob.

Important: The lid should not be opened under pressure because this leads to damage of thelocking mechanism. The respectivefunction is shown in the display. The function of each key can differ from screen to screen. This means that all programparameters are entered into a simple central spreadsheet. Four navigation keys provide easy navigation within the software.

The back and the forward keyhave additional functions as described below This cursor can also be used to complete data entry. By pressing the rightcursor settings will be saved and the cursor moves to the next field. In the file directory, this key moves to cursor forward to the next lower level. The left cursor key moves the cursor back to the previous field. In the file directory, this key moves the cursor back to the higher level. Prior to writing a new program, a program store has to be chosen.

The T Thermocyclerprovides 10 subdirectories of which each can store up to 99 different programs. For easyidentification of the selected storage place, the subdirectories can be named see section.

Use cursor keys to select a subdirectory. The program storages are displayed. EditUsing the softkeys [Page ] and [Page ] you can scroll through the list in 5 program steps. Press [Edit] to write new program in Program store To make retrievalof a program easier, you can enter a name for each program existing of letters, numbers andsymbols.

Blank Del. Name OK EnterUse cursor keys to select a letter. Accept letter with [Enter]. Use cursor keys to select next letter. Repeat until name is completed. Note: Thanks to a new lid design, significantly lower lid temperatures than in the past can beused for thermocycling. The lower lid temperature leads to a higher temperature uniformitywithin the lid and thus to a more even temperature distribution in the tubes.

For instruments featuring the new lid design, a maximum temperature of This is to avoidevaporation during the initial heating phase. EnterNote: The default setting, which is recommended for most applications, is preheating: On. After all pre-settings have been made, press [Enter] to open the programming spreadsheet. In addition, youcan set special parameters like touch down, ramping rates or time increments.

For details onthese special parameters, see section 5. You can navigate in the spreadsheet without limitations with the fourcursor keysNote: Each setting is confirmed with [Enter].

The cursor moves automatically to the next field. Alternatively, you can confirm a value by moving forward with the cursor keys. Note: At any time you can call up the help function with [Info]Note: In an existing protocol program steps can be deleted and inserted. For further informationon deleting and inserting program steps see section 5. Now enter temperature for the first step an press [Enter]. You can also enter any combination of hours, minutes and seconds.

The program data are written to the permanent memory. Cancel Yes NoConfirm changes with [Yes]. The run time is calculated. Enter the desired time increment [seconds] in the row labelled with dt[s].

It supports The flexible design The Mastercycler nexus can accommodate well PCR plates, 0. We are all extremely grateful and can not thank you enough for all that you're doing.

We are wishing you and your family great health during this time. The California Governor has put a "shelter in place" status for all California residents and businesses. However, since we are deemed an "Essential Business" we will remain OPEN to ensure we are able to assist everyone's needs during this fast-spreading Pandemic. The REUSE economy will be instrumental in jump starting the economy, because a lot of new equipment will not be available. Our team can be reached by phone or email at any time.

Our priority is to ensure your questions are answered and your orders are shipped as quickly as possible without compromising the quality of the instrument. We will continue to maintain the highest level of service and support. Onsite service and assistance will be limited at this time to ensure the safety of our employees and others.

If there is a critical issue please contact us via phone or email so we can assist you appropriately. Contact us today Make Offer. Description Specs Description The Biometra T is a Thermocycler that is licensed for PCR applications research use only and intended to be used for performing polymerase chain reactions.

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